5% Irish Cream 6
Hah! It looks like there's a protocol for DNA hybridization in blots using 5% Irish cream liquor! I kid you not. Here's a sample quote from the methods of Yamamoto et al. 1993:
Hybridization was carried out overnight at 65°C in a solution containing 6 × SSC, 5% Irish cream liqueur (Original Irish Cream, R & A Bailey's), 20 mM Na2HPO4, 20 μg/ml heat-denatured salmon sperm DNA, and 2 μCi/ml of the 32P-labelled probe.
The original source appears to be Elbrecht, A. 1987, "Lab Hints: Irish Cream Liqueur as a Blocking Agent for DNA Dot Blots." BM Biochemica, 4:12-13. BM = Boehringer Mannheim, it appears to be a newsletter. It's too obscure for my cursory searching to turn up a copy of the original, I wonder what the motivation was! Maybe this way you can order liquor using grant money? This idea has a lot of potential...!
Comments
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Awesome! You should submit this to NCIROFL.
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so the next time my wife want to buy Baileys, I might be making up a hybridization buffer, with a small sample. BTW lovely graphics on wikipedia (how I found this site) Cheers B
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I might be making up a hybridization buffer, with a small sample. BTW lovely graphics on wikipedia
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