5% Irish Cream 6

Posted by Madeleine Ball Thu, 17 Dec 2009 21:00:00 GMT

Hah! It looks like there's a protocol for DNA hybridization in blots using 5% Irish cream liquor! I kid you not. Here's a sample quote from the methods of Yamamoto et al. 1993:

Hybridization was carried out overnight at 65°C in a solution containing 6 × SSC, 5% Irish cream liqueur (Original Irish Cream, R & A Bailey's), 20 mM Na2HPO4, 20 μg/ml heat-denatured salmon sperm DNA, and 2 μCi/ml of the 32P-labelled probe.

The original source appears to be Elbrecht, A. 1987, "Lab Hints: Irish Cream Liqueur as a Blocking Agent for DNA Dot Blots." BM Biochemica, 4:12-13. BM = Boehringer Mannheim, it appears to be a newsletter. It's too obscure for my cursory searching to turn up a copy of the original, I wonder what the motivation was! Maybe this way you can order liquor using grant money? This idea has a lot of potential...!

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  1. Benjamin Mako Hill 2 days later:
    Awesome! You should submit this to NCIROFL.
  2. Brian Degger 25 days later:
    so the next time my wife want to buy Baileys, I might be making up a hybridization buffer, with a small sample. BTW lovely graphics on wikipedia (how I found this site) Cheers B
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  5. ed hardy uk 8 months later:
    Drink pernicious to health too!
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